Answers::

To Ka Hang and Yvonne (your second question):
The germ tube test is carried out by:
1. Inoculate the yeast into the horse serum.
2. Incubate the serum at 35 – 37oC for 2 - 3 hours.

So, no subculture is needed. We can just do a wet mount directly from the tube. No gram stain will be needed.
3. Do a wet mount using the serum.
4. Observe the slide under the microscope.

To Yvonne (first question):
API is kit that consists of a combination of biochemical test used for identification of organisms. There are different types of API avaliable. For example, for identification of Candida, the API 20C AUX is used. So in conclusion, different types of API kit are used to identify different types of organisms.

To Andika:
To differentiate these Candida species, the same type of API method will be used. But this API intepretation is different from the API method for bacteria.
- For yeast, a change of turbidity will be noted.
- For bacteria, a change in colour will be noted.

To Xin Yi:
About the colour of moulds, i've gotta check it out cos' different moulds have different colours. But i'll find the more common colours for you.

About the cornmeal agar, there is 1% Tween 80 added as a basal medium. This will help to encourage the growth of chlamydospores.


All righty. Hope that I've helped make understanding easier =)!!

-Li Ping-
TG o2